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endogenous gephyrin  (Alomone Labs)


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    Alomone Labs endogenous gephyrin
    Increased multispot nanoscale arrangement <t>of</t> <t>synaptic</t> <t>gephyrin</t> clusters during iLTP. A, B, Pseudocolor representation of SML gephyrin-mEos3.2 normalized local density (radius ∼15 nm) reveals the nanoscale organization of synaptic gephyrin in monospot (A) and multispot (B) clusters. Scale bars, 100 nm. C, Fractions of synaptic clusters organized in monospot or multispot configurations in basal conditions (white bars, n = 120, 51, respectively) and iLTP (gray bars, n = 53, 52, respectively). Data are presented as mean ± SD (D). Molecular characterization [number of molecules (left), area (middle), and density (right)] of monospot and multispot gephyrin synaptic clusters are shown in control (white bars) and during synaptic plasticity (gray bars). Data are presented as median and interquartile range. One-way ANOVA (Kruskal–Wallis test). E, Endogenous and overexpressed gephyrin nanodomains. Left, Representative dual color SML images of gephyrin-mEos3.2 (top, PALM) and endogenous gephyrin (bottom, STORM), imaged along with vGAT-Alexa Fluor 647 and vGAT-Atto520, respectively. Scale bar, 0.5 μm. Right, Magnified normalized local density plots of the synapses framed on the left show the monospot and multispot organization of both gephyrin-mEos3.2 and endogenous gephyrin. Scale bar, 100 nm. F, Number of synaptic endogenous gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP) quantified in STORM. n = 12 and 13 in ctrl and iLTP, respectively. G, Fractions of synaptic clusters organized in monospot and multispot configuration in basal conditions (white bars, n = 147 and 56, respectively) and iLTP (gray bars, n = 81 and 94, respectively) quantified in STORM. Data are presented as mean ± SD, n.s. nonsignificant, *p < 0.05, **p < 0.01.
    Endogenous Gephyrin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous gephyrin/product/Alomone Labs
    Average 92 stars, based on 6 article reviews
    endogenous gephyrin - by Bioz Stars, 2026-02
    92/100 stars

    Images

    1) Product Images from "Nanoscale Molecular Reorganization of the Inhibitory Postsynaptic Density Is a Determinant of GABAergic Synaptic Potentiation"

    Article Title: Nanoscale Molecular Reorganization of the Inhibitory Postsynaptic Density Is a Determinant of GABAergic Synaptic Potentiation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.0514-16.2016

    Increased multispot nanoscale arrangement of synaptic gephyrin clusters during iLTP. A, B, Pseudocolor representation of SML gephyrin-mEos3.2 normalized local density (radius ∼15 nm) reveals the nanoscale organization of synaptic gephyrin in monospot (A) and multispot (B) clusters. Scale bars, 100 nm. C, Fractions of synaptic clusters organized in monospot or multispot configurations in basal conditions (white bars, n = 120, 51, respectively) and iLTP (gray bars, n = 53, 52, respectively). Data are presented as mean ± SD (D). Molecular characterization [number of molecules (left), area (middle), and density (right)] of monospot and multispot gephyrin synaptic clusters are shown in control (white bars) and during synaptic plasticity (gray bars). Data are presented as median and interquartile range. One-way ANOVA (Kruskal–Wallis test). E, Endogenous and overexpressed gephyrin nanodomains. Left, Representative dual color SML images of gephyrin-mEos3.2 (top, PALM) and endogenous gephyrin (bottom, STORM), imaged along with vGAT-Alexa Fluor 647 and vGAT-Atto520, respectively. Scale bar, 0.5 μm. Right, Magnified normalized local density plots of the synapses framed on the left show the monospot and multispot organization of both gephyrin-mEos3.2 and endogenous gephyrin. Scale bar, 100 nm. F, Number of synaptic endogenous gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP) quantified in STORM. n = 12 and 13 in ctrl and iLTP, respectively. G, Fractions of synaptic clusters organized in monospot and multispot configuration in basal conditions (white bars, n = 147 and 56, respectively) and iLTP (gray bars, n = 81 and 94, respectively) quantified in STORM. Data are presented as mean ± SD, n.s. nonsignificant, *p < 0.05, **p < 0.01.
    Figure Legend Snippet: Increased multispot nanoscale arrangement of synaptic gephyrin clusters during iLTP. A, B, Pseudocolor representation of SML gephyrin-mEos3.2 normalized local density (radius ∼15 nm) reveals the nanoscale organization of synaptic gephyrin in monospot (A) and multispot (B) clusters. Scale bars, 100 nm. C, Fractions of synaptic clusters organized in monospot or multispot configurations in basal conditions (white bars, n = 120, 51, respectively) and iLTP (gray bars, n = 53, 52, respectively). Data are presented as mean ± SD (D). Molecular characterization [number of molecules (left), area (middle), and density (right)] of monospot and multispot gephyrin synaptic clusters are shown in control (white bars) and during synaptic plasticity (gray bars). Data are presented as median and interquartile range. One-way ANOVA (Kruskal–Wallis test). E, Endogenous and overexpressed gephyrin nanodomains. Left, Representative dual color SML images of gephyrin-mEos3.2 (top, PALM) and endogenous gephyrin (bottom, STORM), imaged along with vGAT-Alexa Fluor 647 and vGAT-Atto520, respectively. Scale bar, 0.5 μm. Right, Magnified normalized local density plots of the synapses framed on the left show the monospot and multispot organization of both gephyrin-mEos3.2 and endogenous gephyrin. Scale bar, 100 nm. F, Number of synaptic endogenous gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP) quantified in STORM. n = 12 and 13 in ctrl and iLTP, respectively. G, Fractions of synaptic clusters organized in monospot and multispot configuration in basal conditions (white bars, n = 147 and 56, respectively) and iLTP (gray bars, n = 81 and 94, respectively) quantified in STORM. Data are presented as mean ± SD, n.s. nonsignificant, *p < 0.05, **p < 0.01.

    Techniques Used:

    Redistribution of extrasynaptic gephyrin to synaptic compartments during iLTP. A, Top, Wide-field image of gephyrin-mEos3.2 (green) and vGAT (red) along a neuronal dendrite. Note gephyrin clusters juxtaposed to presynaptic vGAT (arrowheads). Bottom, PALM reconstruction of gephyrin-mEos3.2 shown above. Scale bars, 1 μm. B, Representative SML images of gephyrin (white) superimposed upon the wide-field image of the presynaptic vGAT (red). Top, Synaptic clusters are represented in blue. Bottom, Extrasynaptic clusters are shown in orange. Scale bars, 1 μm. C, Quantification of gephyrin number of molecules/cluster, area, and molecule density/cluster quantified at extrasynaptic and synaptic compartments (n = 221 and 85, respectively), Mann–Whitney test. D, Representative dual color SML images of gephyrin (green) and vGAT (red) along neuronal dendrites (solid lines) in control (left) and iLTP (right). Extrasynaptic (empty arrowheads) and synaptic gephyrin juxtaposed to vGAT (plain arrowheads) are shown. Scale bar, 0.5 μm. E, Cumulative distribution of gephyrin number of molecules/cluster (left), cluster area (middle), and molecule density/cluster (right) at extrasynaptic (orange, n = 185–260) and synaptic (blue, 85–107) compartments before (ctrl) and during iLTP. Kolmogorov–Smirnov test. F, APV prevents the gephyrin increase elicited by the iLTP-inducing protocol at extrasynaptic (left, n = 250–386-611, for ctrl, iLTP and iLTP + APV, respectively) and synaptic (right, n = 87–146-157, for ctrl, iLTP and iLTP + APV, respectively) compartments. One-way ANOVA (Kruskal–Wallis test), followed by Dunn's post test. G, Number of synaptic gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP). n = 17 and 23 dendrites in ctrl and iLTP, respectively. Student's t test, n.s. nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001.
    Figure Legend Snippet: Redistribution of extrasynaptic gephyrin to synaptic compartments during iLTP. A, Top, Wide-field image of gephyrin-mEos3.2 (green) and vGAT (red) along a neuronal dendrite. Note gephyrin clusters juxtaposed to presynaptic vGAT (arrowheads). Bottom, PALM reconstruction of gephyrin-mEos3.2 shown above. Scale bars, 1 μm. B, Representative SML images of gephyrin (white) superimposed upon the wide-field image of the presynaptic vGAT (red). Top, Synaptic clusters are represented in blue. Bottom, Extrasynaptic clusters are shown in orange. Scale bars, 1 μm. C, Quantification of gephyrin number of molecules/cluster, area, and molecule density/cluster quantified at extrasynaptic and synaptic compartments (n = 221 and 85, respectively), Mann–Whitney test. D, Representative dual color SML images of gephyrin (green) and vGAT (red) along neuronal dendrites (solid lines) in control (left) and iLTP (right). Extrasynaptic (empty arrowheads) and synaptic gephyrin juxtaposed to vGAT (plain arrowheads) are shown. Scale bar, 0.5 μm. E, Cumulative distribution of gephyrin number of molecules/cluster (left), cluster area (middle), and molecule density/cluster (right) at extrasynaptic (orange, n = 185–260) and synaptic (blue, 85–107) compartments before (ctrl) and during iLTP. Kolmogorov–Smirnov test. F, APV prevents the gephyrin increase elicited by the iLTP-inducing protocol at extrasynaptic (left, n = 250–386-611, for ctrl, iLTP and iLTP + APV, respectively) and synaptic (right, n = 87–146-157, for ctrl, iLTP and iLTP + APV, respectively) compartments. One-way ANOVA (Kruskal–Wallis test), followed by Dunn's post test. G, Number of synaptic gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP). n = 17 and 23 dendrites in ctrl and iLTP, respectively. Student's t test, n.s. nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001.

    Techniques Used: MANN-WHITNEY



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    endogenous gephyrin  (Alomone Labs)
    92
    Alomone Labs endogenous gephyrin
    Increased multispot nanoscale arrangement <t>of</t> <t>synaptic</t> <t>gephyrin</t> clusters during iLTP. A, B, Pseudocolor representation of SML gephyrin-mEos3.2 normalized local density (radius ∼15 nm) reveals the nanoscale organization of synaptic gephyrin in monospot (A) and multispot (B) clusters. Scale bars, 100 nm. C, Fractions of synaptic clusters organized in monospot or multispot configurations in basal conditions (white bars, n = 120, 51, respectively) and iLTP (gray bars, n = 53, 52, respectively). Data are presented as mean ± SD (D). Molecular characterization [number of molecules (left), area (middle), and density (right)] of monospot and multispot gephyrin synaptic clusters are shown in control (white bars) and during synaptic plasticity (gray bars). Data are presented as median and interquartile range. One-way ANOVA (Kruskal–Wallis test). E, Endogenous and overexpressed gephyrin nanodomains. Left, Representative dual color SML images of gephyrin-mEos3.2 (top, PALM) and endogenous gephyrin (bottom, STORM), imaged along with vGAT-Alexa Fluor 647 and vGAT-Atto520, respectively. Scale bar, 0.5 μm. Right, Magnified normalized local density plots of the synapses framed on the left show the monospot and multispot organization of both gephyrin-mEos3.2 and endogenous gephyrin. Scale bar, 100 nm. F, Number of synaptic endogenous gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP) quantified in STORM. n = 12 and 13 in ctrl and iLTP, respectively. G, Fractions of synaptic clusters organized in monospot and multispot configuration in basal conditions (white bars, n = 147 and 56, respectively) and iLTP (gray bars, n = 81 and 94, respectively) quantified in STORM. Data are presented as mean ± SD, n.s. nonsignificant, *p < 0.05, **p < 0.01.
    Endogenous Gephyrin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous gephyrin/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    endogenous gephyrin - by Bioz Stars, 2026-02
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Increased multispot nanoscale arrangement of synaptic gephyrin clusters during iLTP. A, B, Pseudocolor representation of SML gephyrin-mEos3.2 normalized local density (radius ∼15 nm) reveals the nanoscale organization of synaptic gephyrin in monospot (A) and multispot (B) clusters. Scale bars, 100 nm. C, Fractions of synaptic clusters organized in monospot or multispot configurations in basal conditions (white bars, n = 120, 51, respectively) and iLTP (gray bars, n = 53, 52, respectively). Data are presented as mean ± SD (D). Molecular characterization [number of molecules (left), area (middle), and density (right)] of monospot and multispot gephyrin synaptic clusters are shown in control (white bars) and during synaptic plasticity (gray bars). Data are presented as median and interquartile range. One-way ANOVA (Kruskal–Wallis test). E, Endogenous and overexpressed gephyrin nanodomains. Left, Representative dual color SML images of gephyrin-mEos3.2 (top, PALM) and endogenous gephyrin (bottom, STORM), imaged along with vGAT-Alexa Fluor 647 and vGAT-Atto520, respectively. Scale bar, 0.5 μm. Right, Magnified normalized local density plots of the synapses framed on the left show the monospot and multispot organization of both gephyrin-mEos3.2 and endogenous gephyrin. Scale bar, 100 nm. F, Number of synaptic endogenous gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP) quantified in STORM. n = 12 and 13 in ctrl and iLTP, respectively. G, Fractions of synaptic clusters organized in monospot and multispot configuration in basal conditions (white bars, n = 147 and 56, respectively) and iLTP (gray bars, n = 81 and 94, respectively) quantified in STORM. Data are presented as mean ± SD, n.s. nonsignificant, *p < 0.05, **p < 0.01.

    Journal: The Journal of Neuroscience

    Article Title: Nanoscale Molecular Reorganization of the Inhibitory Postsynaptic Density Is a Determinant of GABAergic Synaptic Potentiation

    doi: 10.1523/JNEUROSCI.0514-16.2016

    Figure Lengend Snippet: Increased multispot nanoscale arrangement of synaptic gephyrin clusters during iLTP. A, B, Pseudocolor representation of SML gephyrin-mEos3.2 normalized local density (radius ∼15 nm) reveals the nanoscale organization of synaptic gephyrin in monospot (A) and multispot (B) clusters. Scale bars, 100 nm. C, Fractions of synaptic clusters organized in monospot or multispot configurations in basal conditions (white bars, n = 120, 51, respectively) and iLTP (gray bars, n = 53, 52, respectively). Data are presented as mean ± SD (D). Molecular characterization [number of molecules (left), area (middle), and density (right)] of monospot and multispot gephyrin synaptic clusters are shown in control (white bars) and during synaptic plasticity (gray bars). Data are presented as median and interquartile range. One-way ANOVA (Kruskal–Wallis test). E, Endogenous and overexpressed gephyrin nanodomains. Left, Representative dual color SML images of gephyrin-mEos3.2 (top, PALM) and endogenous gephyrin (bottom, STORM), imaged along with vGAT-Alexa Fluor 647 and vGAT-Atto520, respectively. Scale bar, 0.5 μm. Right, Magnified normalized local density plots of the synapses framed on the left show the monospot and multispot organization of both gephyrin-mEos3.2 and endogenous gephyrin. Scale bar, 100 nm. F, Number of synaptic endogenous gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP) quantified in STORM. n = 12 and 13 in ctrl and iLTP, respectively. G, Fractions of synaptic clusters organized in monospot and multispot configuration in basal conditions (white bars, n = 147 and 56, respectively) and iLTP (gray bars, n = 81 and 94, respectively) quantified in STORM. Data are presented as mean ± SD, n.s. nonsignificant, *p < 0.05, **p < 0.01.

    Article Snippet: Alternatively, for stochastic optical reconstruction microscopy (STORM) experiments, presynaptic terminals were immunoprobed with the anti-vGAT antibody along with the mAb7a antibody (both from Synaptic Systems) to localize endogenous gephyrin or with the anti-GABAA receptor α1 subunit (Alomone), followed by specie-specific secondary antibodies coupled to Atto520 and Alexa Fluor 647 (both from Thermo Fisher Scientific), respectively.

    Techniques:

    Redistribution of extrasynaptic gephyrin to synaptic compartments during iLTP. A, Top, Wide-field image of gephyrin-mEos3.2 (green) and vGAT (red) along a neuronal dendrite. Note gephyrin clusters juxtaposed to presynaptic vGAT (arrowheads). Bottom, PALM reconstruction of gephyrin-mEos3.2 shown above. Scale bars, 1 μm. B, Representative SML images of gephyrin (white) superimposed upon the wide-field image of the presynaptic vGAT (red). Top, Synaptic clusters are represented in blue. Bottom, Extrasynaptic clusters are shown in orange. Scale bars, 1 μm. C, Quantification of gephyrin number of molecules/cluster, area, and molecule density/cluster quantified at extrasynaptic and synaptic compartments (n = 221 and 85, respectively), Mann–Whitney test. D, Representative dual color SML images of gephyrin (green) and vGAT (red) along neuronal dendrites (solid lines) in control (left) and iLTP (right). Extrasynaptic (empty arrowheads) and synaptic gephyrin juxtaposed to vGAT (plain arrowheads) are shown. Scale bar, 0.5 μm. E, Cumulative distribution of gephyrin number of molecules/cluster (left), cluster area (middle), and molecule density/cluster (right) at extrasynaptic (orange, n = 185–260) and synaptic (blue, 85–107) compartments before (ctrl) and during iLTP. Kolmogorov–Smirnov test. F, APV prevents the gephyrin increase elicited by the iLTP-inducing protocol at extrasynaptic (left, n = 250–386-611, for ctrl, iLTP and iLTP + APV, respectively) and synaptic (right, n = 87–146-157, for ctrl, iLTP and iLTP + APV, respectively) compartments. One-way ANOVA (Kruskal–Wallis test), followed by Dunn's post test. G, Number of synaptic gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP). n = 17 and 23 dendrites in ctrl and iLTP, respectively. Student's t test, n.s. nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: The Journal of Neuroscience

    Article Title: Nanoscale Molecular Reorganization of the Inhibitory Postsynaptic Density Is a Determinant of GABAergic Synaptic Potentiation

    doi: 10.1523/JNEUROSCI.0514-16.2016

    Figure Lengend Snippet: Redistribution of extrasynaptic gephyrin to synaptic compartments during iLTP. A, Top, Wide-field image of gephyrin-mEos3.2 (green) and vGAT (red) along a neuronal dendrite. Note gephyrin clusters juxtaposed to presynaptic vGAT (arrowheads). Bottom, PALM reconstruction of gephyrin-mEos3.2 shown above. Scale bars, 1 μm. B, Representative SML images of gephyrin (white) superimposed upon the wide-field image of the presynaptic vGAT (red). Top, Synaptic clusters are represented in blue. Bottom, Extrasynaptic clusters are shown in orange. Scale bars, 1 μm. C, Quantification of gephyrin number of molecules/cluster, area, and molecule density/cluster quantified at extrasynaptic and synaptic compartments (n = 221 and 85, respectively), Mann–Whitney test. D, Representative dual color SML images of gephyrin (green) and vGAT (red) along neuronal dendrites (solid lines) in control (left) and iLTP (right). Extrasynaptic (empty arrowheads) and synaptic gephyrin juxtaposed to vGAT (plain arrowheads) are shown. Scale bar, 0.5 μm. E, Cumulative distribution of gephyrin number of molecules/cluster (left), cluster area (middle), and molecule density/cluster (right) at extrasynaptic (orange, n = 185–260) and synaptic (blue, 85–107) compartments before (ctrl) and during iLTP. Kolmogorov–Smirnov test. F, APV prevents the gephyrin increase elicited by the iLTP-inducing protocol at extrasynaptic (left, n = 250–386-611, for ctrl, iLTP and iLTP + APV, respectively) and synaptic (right, n = 87–146-157, for ctrl, iLTP and iLTP + APV, respectively) compartments. One-way ANOVA (Kruskal–Wallis test), followed by Dunn's post test. G, Number of synaptic gephyrin clusters per dendrite area under basal conditions (ctrl) and during synaptic plasticity (iLTP). n = 17 and 23 dendrites in ctrl and iLTP, respectively. Student's t test, n.s. nonsignificant, *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: Alternatively, for stochastic optical reconstruction microscopy (STORM) experiments, presynaptic terminals were immunoprobed with the anti-vGAT antibody along with the mAb7a antibody (both from Synaptic Systems) to localize endogenous gephyrin or with the anti-GABAA receptor α1 subunit (Alomone), followed by specie-specific secondary antibodies coupled to Atto520 and Alexa Fluor 647 (both from Thermo Fisher Scientific), respectively.

    Techniques: MANN-WHITNEY